Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments. (22nd February 2021)

• Record Type: Journal Article
• Authors: Highfield, Andrea; Ward, Angela; Pipe, Richard; Schroeder, Declan C.
• Is Part Of: Journal of the Marine Biological Association of the United Kingdom. Volume 101:Number 1(2021)
• Page Start: 27
• Page End: 37
• Publication Date: 2021-02-22
• Subjects: Diversity, -- Dunaliella salina, -- ITS, -- LSU, -- phylogeny, -- rbcL, -- SSU, -- tufA; Biology -- Periodicals; Marine biology -- Periodicals; 578.77
• DOI: 10.1017/S0025315420001319
• Languages: English
• ISSNs: 0025-3154

Abstract

Abstract: Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbc L and tuf A and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina . We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.