Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato  . Issue 10 (31st October 2014)

• Record Type: Journal Article
• Authors: Chen, Ying-Lan; Lee, Chi-Ying; Cheng, Kai-Tan; Chang, Wei-Hung; Huang, Rong-Nan; Nam, Hong Gil; Chen, Yet-Ran
• Is Part Of: The Plant Cell. Volume 26:Issue 10(2014)
• Page Start: 4135
• Page End: 4148
• Publication Date: 2014-10-31
• DOI: 10.1105/tpc.114.131185
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : CAPE1, a conserved peptide elicitor derived from tomato PR-1, was induced by wounding and found to regulate immune responses against biological threats. As PR-1 is highly conserved across many organisms and the putative peptide from AtPR1 was also found to be bioactive in Arabidopsis, the results suggest that this peptide may be useful for enhancing resistance to stress in other plant species. Abstract: Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato ( Solanum lycopersicum ) and quantified to be induced by both wounding and methyl jasmonate (MeJA ). A wounding or wounding plus MeJA -induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.