The Arabidopsis Malectin-Like/LRR-RLK IOS1 Is Critical for BAK1-Dependent and BAK1-Independent Pattern-Triggered Immunity. Issue 7 (17th June 2016)

• Record Type: Journal Article
• Authors: Yeh, Yu-Hung; Panzeri, Dario; Kadota, Yasuhiro; Huang, Yi-Chun; Huang, Pin-Yao; Tao, Chia-Nan; Roux, Milena; Chien, Hsiao-Chiao; Chin, Tzu-Chuan; Chu, Po-Wei; Zipfel, Cyril; Zimmerli, Laurent
• Is Part Of: The Plant Cell. Volume 28:Issue 7(2016)
• Page Start: 1701
• Page End: 1721
• Publication Date: 2016-06-17
• DOI: 10.1105/tpc.16.00313
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : The receptor-like kinase IOS1 is critical for priming of Arabidopsis thaliana innate immunity and is required for optimal function of BAK1-dependent and BAK1-independent cell surface immune receptors. Abstract: Plasma membrane-localized pattern recognition receptors (PRRs) such as FLAGELLIN SENSING2 (FLS2), EF-TU RECEPTOR (EFR), and CHITIN ELICITOR RECEPTOR KINASE1 (CERK1) recognize microbe-associated molecular patterns (MAMPs) to activate pattern-triggered immunity (PTI ). A reverse genetics approach on genes responsive to the priming agent β-aminobutyric acid (BABA ) revealed IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) as a critical PTI player. Arabidopsis thaliana ios1 mutants were hypersusceptible to Pseudomonas syringae bacteria. Accordingly, ios1 mutants showed defective PTI responses, notably delayed upregulation of the PTI marker gene FLG22-INDUCED RECEPTOR-LIKE KINASE1, reduced callose deposition, and mitogen-activated protein kinase activation upon MAMP treatment. Moreover, Arabidopsis lines overexpressing IOS1 were more resistant to bacteria and showed a primed PTI response. In vitro pull-down, bimolecular fluorescence complementation, coimmunoprecipitation, and mass spectrometry analyses supported the existence of complexes between the membrane-localized IOS1 and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1)-dependent PRRs FLS2 and EFR, as well as with the BAK1-independent PRR CERK1. IOS1 also associated with BAK1 in a ligand-independent manner and positively regulated FLS2-BAK1 complex formation upon MAMP treatment. In addition, IOS1 was critical for chitin-mediated PTI. Finally, ios1 mutants were defective in BABA -induced resistance and priming. This work reveals IOS1 as a novel regulatory protein of FLS2-, EFR-, and CERK1-mediated signaling pathways that primes PTI activation.