ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes. Issue 11 (15th November 2016)

• Record Type: Journal Article
• Authors: Zinsmeister, Julia; Lalanne, David; Terrasson, Emmanuel; Chatelain, Emilie; Vandecasteele, Céline; Vu, Benoit Ly; Dubois-Laurent, Cécile; Geoffriau, Emmanuel; Signor, Christine Le; Dalmais, Marion; Gutbrod, Katharina; Dörmann, Peter; Gallardo, Karine; Bendahmane, Abdelhafid; Buitink, Julia; Leprince, Olivier
• Is Part Of: The Plant Cell. Volume 28:Issue 11(2016)
• Page Start: 2735
• Page End: 2754
• Publication Date: 2016-11-15
• DOI: 10.1105/tpc.16.00470
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : ABI5 is a prominent regulator of late seed maturation in legumes, coupling seed vigor with raffinose family oligosaccharide and late embryogenesis abundant protein accumulation and degreening. Abstract: The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage. In a quantitative trait locus analysis on seed longevity in Medicago truncatula, we identified the bZIP transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5). Characterization of Mt- abi5 insertion mutant seeds revealed that both the acquisition of longevity and dormancy were severely impaired. Using transcriptomes of developing Mt-abi5 seeds, we created a gene coexpression network and revealed ABI5 as a regulator of gene modules with functions related to raffinose family oligosaccharide (RFO ) metabolism, late embryogenesis abundant (LEA ) proteins, and photosynthesis-associated nuclear genes (PhANGs). Lower RFO contents in Mt-abi5 seeds were linked to the regulation of SEED IMBIBITION PROTEIN1 . Proteomic analysis confirmed that a set of LEA polypeptides was reduced in mature Mt -abi5 seeds, whereas the absence of repression of PhANG in mature Mt -abi5 seeds was accompanied by chlorophyll and carotenoid retention. This resulted in a stress response in Mt -abi5 seeds, evident from an increase in α-tocopherol and upregulation of genes related to programmed cell death and protein folding. Characterization of abi5 mutants in a second legume species, pea ( Pisum sativum ), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes.