In Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlings. Issue 10 (11th October 2016)

• Record Type: Journal Article
• Authors: Reichel, Marlene; Liao, Yalin; Rettel, Mandy; Ragan, Chikako; Evers, Maurits; Alleaume, Anne-Marie; Horos, Rastislav; Hentze, Matthias W.; Preiss, Thomas; Millar, Anthony A.
• Is Part Of: The Plant Cell. Volume 28:Issue 10(2016)
• Page Start: 2435
• Page End: 2452
• Publication Date: 2016-10-11
• DOI: 10.1105/tpc.16.00562
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : The mRNA-interactome capture method, which utilizes UV light to cross-link proteins and mRNA in planta, was used to identify the portion of the proteome that binds RNA in etiolated Arabidopsis seedlings. Abstract: RNA binding proteins (RBPs) control the fate and expression of a transcriptome. Despite this fundamental importance, our understanding of plant RBPs is rudimentary, being mainly derived via bioinformatic extrapolation from other kingdoms. Here, we adapted the mRNA-protein interactome capture method to investigate the RNA binding proteome in planta. From Arabidopsis thaliana etiolated seedlings, we captured more than 700 proteins, including 300 with high confidence that we have defined as the At-RBP set. Approximately 75% of these At-RBPs are bioinformatically linked with RNA biology, containing a diversity of canonical RNA binding domains (RBDs ). As no prior experimental RNA binding evidence exists for the majority of these proteins, their capture now authenticates them as RBPs. Moreover, we identified protein families harboring emerging and potentially novel RBDs, including WHIRLY, LIM, ALBA, DUF1296, and YTH domain-containing proteins, the latter being homologous to animal RNA methylation readers. Other At-RBP set proteins include major signaling proteins, cytoskeleton-associated proteins, membrane transporters, and enzymes, suggesting the scope and function of RNA-protein interactions within a plant cell is much broader than previously appreciated. Therefore, our foundation data set has provided an unbiased insight into the RNA binding proteome of plants, on which future investigations into plant RBPs can be based.