GIF Transcriptional Coregulators Control Root Meristem Homeostasis. Issue 2 (18th January 2018)

• Record Type: Journal Article
• Authors: Ercoli, María Florencia; Ferela, Antonella; Debernardi, Juan Manuel; Perrone, Ana Paula; Rodriguez, Ramiro E.; Palatnik, Javier F.
• Is Part Of: The Plant Cell. Volume 30:Issue 2(2018)
• Page Start: 347
• Page End: 359
• Publication Date: 2018-01-18
• DOI: 10.1105/tpc.17.00856
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : GRF-INTERACTING FACTORs, including ANGUSTIFOLIA3, GIF2, and GIF3, control root meristem and quiescent center organization through interactions with GRF transcription factors and BRAHMA complexes. Abstract: In the root meristem, the quiescent center (QC) is surrounded by stem cells, which in turn generate the different cell types of the root. QC cells rarely divide under normal conditions but can replenish damaged stem cells. In the proximal meristem, the daughters of stem cells, which are referred to as transit-amplifying cells, undergo additional rounds of cell division prior to differentiation. Here, we describe the functions of GRF-INTERACTING FACTORs ( GIFs ), including ANGUSTIFOLIA3 ( AN3 ), in Arabidopsis thaliana roots. GIFs have been shown to interact with GRF transcription factors and SWI/SNF chromatin remodeling complexes. We found that combinations of GIF mutants cause the loss of QC identity. However, despite their QC impairment, GIF mutants have a significantly enlarged root meristem with additional lateral root cap layers. We show that the increased expression of PLETHORA1 ( PLT1 ) is at least partially responsible for the large root meristems of an3 mutants. Furthermore, we found that GIFs are necessary for maintaining the precise expression patterns of key developmental regulators and that AN3 complexes bind directly to the promoter regions of PLT1 as well as SCARECROW . We propose that AN3/GIFs participate in different pathways that control QC organization and the size of the meristem.