Neural priming of adipose-derived stem cells by cell-imprinted substrates*. (2nd April 2021)

• Record Type: Journal Article
• Authors: Ghazali, Zahra Sadat; Eskandari, Mahnaz; Bonakdar, Shahin; Renaud, Philippe; Mashinchian, Omid; Shalileh, Shahriar; Bonini, Fabien; Uckay, Ilker; Preynat-Seauve, Olivier; Braschler, Thomas
• Is Part Of: Biofabrication. Volume 13:Number 3(2021)
• Publication Date: 2021-04-02
• Subjects: neural tissue engineering -- cell imprinting -- human mesenchymal stem cells -- patterned substrate -- neural differentiation; Biomedical engineering -- Periodicals; Tissue engineering -- Periodicals; Biomedical materials -- Microstructure -- Periodicals; Bioengineering -- Periodicals; 610.28
• DOI: 10.1088/1758-5090/abc66f
• Languages: English
• ISSNs: 1758-5082

Abstract

Abstract: Cell-imprinting technology is a novel method for directing stem cell fate using substrates molded from target cells. Here, we fabricated and studied cell-imprinted substrates for neural priming in human adipose-derived stem cells in the absence of chemical cues. We molded polydimethylsiloxane silicone substrates on fixed differentiated neural progenitor cells (ReNcell TM VM). The ReNcell TM cell line consists of immortalized human neural progenitor cells that are capable to differentiate into neural cells. The fabricated cell-imprinted silicone substrates represent the geometrical micro- and nanotopology of the target cell morphology. During the molding procedure, no transfer of cellular proteins was detectable. In the first test with undifferentiated ReNcell TM VM cells, the cell-imprinted substrates could accelerate neural differentiation. With adipose-derived stem cells cultivated on the imprinted substrates, we observed modifications of cell morphology, shifting from spread to elongated shape. Both immunofluorescence and quantitative gene expression analysis showed upregulation of neural stem cell and early neuronal markers. Our study, for the first time, demonstrated the effectiveness of cell-imprinted substrates for neural priming of adipose-derived stem cells for regenerative medicine applications.