Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis. (27th March 2017)

• Record Type: Journal Article
• Authors: Thwaites, Ryan S.; Ito, Kazuhiro; Chingono, Jasmine M. S.; Coates, Matthew; Jarvis, Hannah C.; Tunstall, Tanushree; Anderson-Dring, Lauren; Cass, Lindsey; Rapeport, Garth; Openshaw, Peter J.; Nadel, Simon; Hansel, Trevor T.
• Is Part Of: Journal of infectious diseases. Volume 215:Number 8(2017:Apr. 15)
• Page Start: 1240
• Page End: 1244
• Publication Date: 2017-03-27
• Subjects: respiratory syncytial virus -- nasosorption -- viral load -- bronchiolitis -- respiratory sampling.; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jix150
• Languages: English
• ISSNs: 0022-1899

Abstract

Summary: We used nasosorption as a noninvasive method of respiratory mucosal sampling in a paediatric virology setting. Nasosorption was well tolerated and demonstrated respiratory syncytial virus load to be associated with disease severity and elevated mediators of inflammation. Abstract: Background: Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting. Methods: Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA). Results: Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay ( P = .04) and requirement for mechanical ventilation ( P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load ( P < .05). Conclusions: Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.