Alleviation of heavy metal and silver nanoparticle toxicity and enhancement of their removal by hydrogen sulfide in Phanerochaete chrysosporium. (June 2019)

• Record Type: Journal Article
• Authors: Huang, Zhenzhen; He, Kai; Song, Zhongxian; Zeng, Guangming; Chen, Anwei; Yuan, Lei; Li, Hui; Chen, Guiqiu
• Is Part Of: Chemosphere. Volume 224(2019)
• Page Start: 554
• Page End: 561
• Publication Date: 2019-06
• Subjects: Hydrogen sulfide -- Heavy metals -- Silver nanoparticles -- Bioremediation -- Antioxidant response -- Phanerochaete chrysosporium; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.02.190
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Hydrogen sulfide (H2 S), an important cellular signaling molecule, plays vital roles in mediating responses to biotic/abiotic stresses. Influences of H2 S on metal removal, cell viability, and antioxidant response of Phanerochaete chrysosporium upon exposure to heavy metals and silver nanoparticles (AgNPs) in the present study were investigated. An enhancement in Pb(ΙΙ) removal with an increase in concentration of the H2 S donor sodium hydrosulfide (NaHS) was observed, and the maximum removal efficiencies increased by 31% and 17% under 100 and 200 mg/L Pb(ΙΙ) exposure, respectively, in the presence of 500 μM NaHS. Application of 500 μM NaHS increased the cell viability by 15%–39% under Pb(II) stress (10–200 mg/L) with relative to the untreated control. Increase in total Ag uptake and cell survival was also elicited by NaHS in a concentration-dependent manner under AgNP stress. Meanwhile, activities of superoxide dismutase and catalase were significantly enhanced with the introduction of NaHS under stresses of Pb(II), Cd(II), Cu(II), Zn(II), Ni(II), and AgNPs. The inhibition in lipid peroxidation and oxidative stress was observed in P. chrysosporium cells exposed to these toxicants following NaHS pretreatment, which could be attributed to the upregulation in antioxidant enzymes. The results obtained suggest that H2 S can alleviate heavy metals and AgNP-induced toxicity to P. chrysosporium and improve the removal efficiency of these toxicants from wastewater. Highlights: H2 S can greatly improve the removal of metal ions and total Ag from wastewater. A dose-dependent increase in cell survival was evoked by H2 S under toxicant stress. Application of H2 S significantly enhanced the expression of SOD and CAT activities. MDA and O2 – levels were alleviated in P. chrysosporium cells pretreated with H2 S. Inhibition in oxidative stress was ascribed to upregulation in antioxidant enzymes.