Testicular endothelial cells promote self-renewal of spermatogonial stem cells in rats†. Issue 2 (11th June 2019)

• Record Type: Journal Article
• Authors: Kim, Yong-Hee; Oh, Myeong-Geun; Bhang, Dong Ha; Kim, Bang-Jin; Jung, Sang-Eun; Kim, Seok-Man; Dohr, Gottfried; Kim, Sun-Uk; Ryeom, Sandra; Ryu, Buom-Yong
• Is Part Of: Biology of reproduction. Volume 101:Issue 2(2019)
• Page Start: 360
• Page End: 367
• Publication Date: 2019-06-11
• Subjects: rat -- spermatogonial stem cell -- endothelial cell -- in vitro culture -- transplantation; Reproduction -- Periodicals; Biology; Reproduction; Reproduction; Electronic journals; Periodicals; Periodicals; 571.805
• DOI: 10.1093/biolre/ioz105
• Languages: English
• ISSNs: 0006-3363

Abstract

Abstract: Spermatogonial stem cells (SSCs) are the basis of spermatogenesis in male due to their capability to multiply in numbers by self-renewal and subsequent meiotic processes. However, as SSCs are present in a very small proportion in the testis, in vitro proliferation of undifferentiated SSCs will facilitate the study of germ cell biology. In this study, we investigated the effectiveness of various cell lines as a feeder layer for rat SSCs. Germ cells enriched for SSCs were cultured on feeder layers including SIM mouse embryo-derived thioguanine and ouabain-resistant cells, C166 cells, and mouse and rat testicular endothelial cells (TECs) and their stem cell potential for generating donor-derived colonies and offspring was assessed by transplantation into recipient testes. Rat germ cells cultured on TECs showed increased mRNA and protein levels of undifferentiated spermatogonial markers. Rat SSCs derived from these germ cells underwent spermatogenesis and generated offspring when transplanted into recipients. Collectively, TECs can serve as an effective feeder layer that enhances the proliferative and self-renewal capacity of cultured rat SSCs while preserving their stemness properties. Abstract : TECs are effective as feeder cells for maintaining the stemness of spermatogonial stem cells that can undergo spermatogenesis and generate offspring.