Environmental dissemination of mcr-1 positive Enterobacteriaceae by Chrysomya spp. (common blowfly): An increasing public health risk. (January 2019)

• Record Type: Journal Article
• Authors: Yang, Qiu E.; Tansawai, Uttapoln; Andrey, Diego O.; Wang, Shaolin; Wang, Yang; Sands, Kirsty; Kiddee, Anong; Assawatheptawee, Kanit; Bunchu, Nophawan; Hassan, Brekhna; Walsh, Timothy Rutland; Niumsup, Pannika R.
• Is Part Of: Environment international. Volume 122(2019)
• Page Start: 281
• Page End: 290
• Publication Date: 2019-01
• Subjects: Blow flies -- mcr-1 gene -- Klebsiella pneumoniae -- IncX4 plasmid -- Multidrug resistance; Environmental protection -- Periodicals; Environmental health -- Periodicals; Environmental monitoring -- Periodicals; Environmental Monitoring -- Periodicals; Environnement -- Protection -- Périodiques; Hygiène du milieu -- Périodiques; Environnement -- Surveillance -- Périodiques; Environmental health; Environmental monitoring; Environmental protection; Periodicals; 333.705
• DOI: 10.1016/j.envint.2018.11.021
• Languages: English
• ISSNs: 0160-4120

Abstract

Abstract: Until recently, the role of insects, and particularly flies, in disseminating antimicrobial resistance (AMR) has been poorly studied. In this study, we screened blowflies ( Chrysomya spp.) from different areas near the city of Phitsanulok, Northern Thailand, for the presence of AMR genes and in particular, mcr-1, using whole genome sequencing (WGS). In total, 48 mcr-1 -positive isolates were recovered, consisting of 17 mcr-1 -positive Klebsiella pneumoniae (MCRPKP) and 31 mcr-1 -positive Escherichia coli (MCRPEC) strains. The 17 MCRPKP were shown to be clonal (ST43) with few single poly nucleomorphs (SNPs) by WGS analysis. In in-vitro models, the MCRPKP were shown to be highly virulent. In contrast, 31 recovered MCRPEC isolates are varied, belonging to 12 different sequence types shared with those causing human infections. The majority of mcr-1 gene are located on IncX4 plasmids (29/48, 60.42%), sharing an identical plasmid backbone. These findings highlight the contribution of flies to the AMR contagion picture in low- and middle-income countries and the challenges of tackling global AMR. Highlights: WGS data of MCRPE isolates suggests flies are active vectors for the environmental spread of colistin resistant pathogens. Clonal, virulent MCRPKP isolates were identified from blowflies, which post an acute public health risk. Diversity of AMR genes was detected in bacteria from flies indicating the environmental spread of MDR pathogens Highly identical plasmid backbones were observed among mcr-1 -harboring IncX4 plasmids obtained from blowflies.