Full length obtains of hydA and phylogenetic analysis of bio-hydrogen production new species of Clostridium based on efficient hydA degenerate primers. (12th November 2019)

• Record Type: Journal Article
• Authors: Zhao, Xin; Wang, Zhiqing; Zhou, Xiuyan; Qi, Nan; Chen, Fang; Li, Danyang; Li, Xuejie
• Is Part Of: International journal of hydrogen energy. Volume 44:Number 56(2019)
• Page Start: 29493
• Page End: 29499
• Publication Date: 2019-11-12
• Subjects: Biohydrogen -- [FeFe]-hydrogenase -- Clostridium -- Degenerate primer -- Phylogenetic analysis; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2019.05.050
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: In fermentative bio-hydrogen production, bacteria from genus Clostridium are considered to be efficient candidates. Studies on bacteria isolation and functional gene analysis of Clostridium make contribution to the H2 production efficiency improvement. In this study, six degenerate primers were designed based on [FeFe]-hydrogenase ( hyd A) gene sequences from representative biohydrogen producers of Clostridium, and employed to obtain the hyd A gene open reading frames (ORFs) of C . guangxiense and C . neuense . Their phylogenetic relationships were also evaluated with hyd A gene partial sequences. The ORFs of hyd A gene from strains C . guangxiense ZGM211 T and C . neuense G1 T were obtained with the same length of 1719 bp and theoretical pI 8.09, but different stop codons of TAA and TAG, and Mw of 63.03 and 63.13 kDa, respectively. The phylogenetic tree constructed with hyd A partial gene sequences was very similar to that of full-length 16S rRNA gene. It more accurately reflected the phylogenetic relationship of C . guangxiense, C . neuense and related species, and suggested that the hyd A gene could be an assistant or reference to 16S rRNA gene in the phylogenetic relationship investigation of bio-hydrogen producers within genus Clostridium . Highlights: Degenerate primers for hyd A of Clostrdium within different study purposes in bio-hydrogen production were designed. Full-length hyd As from new H2 production species of Clostridium were obtained. Phylogenetic relationship of species within Clostridium was accurately reflected by hyd A gene. The hyd A is good assistant for 16S rRNA gene in the phylogenetic investigation of Clostridium.