A myxobacterial LPMO10 has oxidizing cellulose activity for promoting biomass enzymatic saccharification of agricultural crop straws. (December 2020)

• Record Type: Journal Article
• Authors: Zhou, Xiaoli; Xu, Zhiqiang; He, Jia; Li, Yueqiu; Pan, Chengxiang; Wang, Chunling; Deng, Ming-Rong; Zhu, Honghui
• Is Part Of: Bioresource technology. Volume 318(2020)
• Publication Date: 2020-12
• Subjects: LPMO10 -- Myxobacteria -- Agricultural residual biomass -- Cellulase cocktail; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124217
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: ViLPMO10B is a myxobacterial C1-oxidizing cellulose-active LPMO. ViLPMO10B synergyzes with commercial cellulase in degradation of crop straws. ViLPMO10B reduces the degree of polymerization of crop straw cellulose. ViLPMO10B has application potential in the utilization of agricultural waste. Abstract: Myxobacteria are soil microorganisms with the ability to break down biological macromolecules due to the secretion of a large number of extracellular enzymes, but there has been no research report on myxobacterial lytic polysaccharide monooxygenases (LPMOs). In this study, two LPMO10s, ViLPMO10A and ViLPMO10B, from myxobacterium Vitiosangium sp. GDMCC 1.1324 were characterized. Of which, ViLPMO10B is a C1-oxidizing cellulose-active LPMO. Moreover, ViLPMO10B could decrease the degree of polymerization of crop straws cellulose and synergize with commercial cellulase to promote the saccharification. When the weight ratio of commercial cellulase to ViLPMO10B was 9:1, the conversion efficiency of corn stalk, sugarcane bagasse, and rice straw into reducing sugar was improved by 17%, 16%, and 22%, respectively, compared with commercial cellulase without ViLPMO10B. These results indicate that ViLPMO10B has the potential to be a component of a high-efficient cellulase cocktail and has application value in the saccharification of agricultural residual biomasses.