Growth of high-strength protein crystals with nanofibers. (9th February 2016)

• Record Type: Journal Article
• Authors: Matsuoka, Miki; Kakinouchi, Keisuke; Adachi, Hiroaki; Maruyama, Mihoko; Sugiyama, Shigeru; Nakabayashi, Iori; Tsuchikura, Hiroshi; Kuwahara, Atsushi; Sano, Satoshi; Yoshikawa, Hiroshi Y.; Takahashi, Yoshinori; Yoshimura, Masashi; Matsumura, Hiroyoshi; Murakami, Satoshi; Inoue, Tsuyoshi; Mori, Yusuke; Takano, Kazufumi
• Is Part Of: Applied physics express. Volume 9:Number 3(2016:Mar.)
• Publication Date: 2016-02-09
• Subjects: Physics -- Periodicals; Technology -- Periodicals; 621.05
• DOI: 10.7567/APEX.9.035503
• Languages: English
• ISSNs: 1882-0778

Abstract

Abstract: Here, we present a novel method of growing protein crystals with nanofibers. Protein crystals were grown by incorporating nanofibers. No obvious differences were observed in diffraction data between fiber-containing and as-grown crystals. The fiber-containing crystals displayed an increased tolerance to osmotic shock caused by soaking in 25% ethanol or 40% dimethyl sulfoxide. This means that the method allowed us to easily increase the crystal mechanical stability. Because the method is very simple, it will provide a variety of possibilities for protein crystallization.