A new approach to bioanalysis: aqueous normal-phase chromatography with silica hydride stationary phases. (April 2012)

• Record Type: Journal Article
• Authors: Pesek, Joseph J; Matyska, Maria T
• Is Part Of: Bioanalysis. Volume 4:Number 7(2012)
• Page Start: 845
• Page End: 853
• Publication Date: 2012-04
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.12.39
• Languages: English
• ISSNs: 1757-6180

Abstract

Stationary phases based on silica hydride have demonstrated a number of unique properties that are especially advantageous for bioanalyses. They have excellent retention capabilities for hydrophilic compounds, which have been the most difficult to analyze by standard reversed-phase methods and, in many cases, can outperform newer approaches for the analysis of polar molecules, such as hydrophilic liquid interaction chromatography. In addition, all columns utilizing silica-hydride materials can be used in either the normal-phase or reversed-phase modes, sometimes retaining both polar and nonpolar compounds simultaneously. These stationary phases have a high degree of reproducibility and long-term stability.