MpTCP1 controls cell proliferation and redox processes in Marchantia polymorpha. Issue 4 (6th October 2019)

• Record Type: Journal Article
• Authors: Busch, Andrea; Deckena, Marek; Almeida‐Trapp, Marilia; Kopischke, Sarah; Kock, Cilian; Schüssler, Esther; Tsiantis, Miltos; Mithöfer, Axel; Zachgo, Sabine
• Is Part Of: New phytologist. Volume 224:Issue 4(2019)
• Page Start: 1627
• Page End: 1641
• Publication Date: 2019-10-06
• Subjects: cell proliferation -- DNA binding -- evolution -- Marchantia polymorpha -- redox regulation -- secondary metabolites -- TCP; Botany -- Periodicals; 580
• DOI: 10.1111/nph.16132
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: TCP transcription factors are key regulators of angiosperm cell proliferation processes. It is unknown whether their regulatory growth capacities are conserved across land plants, which we examined in liverworts, one of the earliest diverging land plant lineages. We generated knockout mutants for Mp TCP1, the single TCP‐P clade gene in Marchantia polymorpha, and characterized its function by conducting cell proliferation and morphological analyses as well as messenger RNA expression, transcriptome, chemical, and DNA binding studies. Mp tcp1 ge lines show a reduced vegetative thallus growth and extra tissue formation in female reproductive structures. Additionally, mutant plants reveal increased hydrogen peroxide (H2 O2 ) levels and an enhanced pigmentation in the thallus caused by formation of secondary metabolites, such as aminochromes. MpTCP1 proteins interact redox dependently with DNA and regulate the expression of a comprehensive redox network, comprising enzymes involved in H2 O2 metabolism. Mp TCP1 regulates Marchantia growth in a context‐dependent manner. Redox sensitivity of the DNA binding capacity of MpTCP1 proteins provides a mechanism to respond to altered redox conditions. Our data suggest that Mp TCP1 activity could thereby have contributed to diversification of land plant morphologies and to adaptations to abiotic and biotic challenges, as experienced by liverworts during early land plant colonization.