Unidirectional Presentation of Membrane Proteins in Nanoparticle‐Supported Liposomes. Issue 29 (7th May 2019)

• Record Type: Journal Article
• Authors: Chen, Wen; Cai, Yongfei; Fu, Qingshan; Chen, Bing; Guo, Junling; Chou, James J.
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 29(2019)
• Page Start: 9866
• Page End: 9870
• Publication Date: 2019-05-07
• Subjects: HIV-1 MPER -- membrane antigen presentation -- nanoparticles -- proteoliposome formation -- unidirectionality; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201903093
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Presentation of membrane proteins to host immune systems has been a challenging problem owing to complexity arising from the poor in vivo stability of the membrane‐mimetic media often used for solubilizing the membrane proteins. The use of functionalized, biocompatible nanoparticles as substrates is shown to guide the formation of proteoliposomes, which can present many copies of membrane proteins in a unidirectional manner. The approach was demonstrated to present the membrane‐proximal region of the HIV‐1 envelope glycoprotein. These nanoparticle‐supported liposomes are broadly applicable as membrane antigen vehicles for inducing host immune responses. Abstract : It's SPLANDID : Solid and hollow nanoparticles can both be used as core substrates to recruit membrane proteins in bicelles through affinity interaction. As detergent is removed, the bicelles will merge and form proteoliposomes encapsulating the nanoparticles, presenting high copy numbers of membrane proteins in a unidirectional manner.