A DNAzyme‐Based Colorimetric Paper Sensor for Helicobacter pylori. Issue 29 (6th June 2019)

• Record Type: Journal Article
• Authors: Ali, M. Monsur; Wolfe, Michael; Tram, Kha; Gu, Jimmy; Filipe, Carlos D. M.; Li, Yingfu; Brennan, John D.
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 29(2019)
• Page Start: 9907
• Page End: 9911
• Publication Date: 2019-06-06
• Subjects: biosensors -- DNAzymes -- Helicobacter pylori -- paper analytical devices; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201901873
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The reliable detection of pathogenic bacteria in complex biological samples using simple assays or devices remains a major challenge. Herein, we report a simple colorimetric paper device capable of providing specific and sensitive detection of Helicobacter pylori (H. pylori), a pathogen strongly linked to gastric carcinoma, gastric ulcers, and duodenal ulcers, in stool samples. The sensor molecule, an RNA‐cleaving DNAzyme obtained through in vitro selection, is activated by a protein biomarker from H. pylori . The colorimetric paper sensor, designed on the basis of the RNA‐cleaving property of the DNAzyme, is capable of sensitive detection of H. pylori in human stool samples with minimal sample processing and provides results in minutes. It remains fully functional under storage at ambient temperature for at least 130 days. This work lays a foundation for developing DNAzyme‐enabled paper‐based point‐of‐care diagnostic devices for monitoring pathogens in complex samples. Abstract : A litmus test for bacteria : A highly selective and sensitive paper‐based sensor for the detection of Helicobacter pylori (HP) in human stool samples is presented. The sensor produces a colorimetric signal in minutes and is stable for several months, allowing non‐invasive testing of HP in resource‐limited areas.