Elevated temperature overrides the effects of N amendment in Tibetan grassland on soil microbiome. (September 2019)

• Record Type: Journal Article
• Authors: Wang, Zhujun; Lu, Guangxin; Yuan, Mengting; Yu, Hao; Wang, Shang; Li, Xin; Deng, Ye
• Is Part Of: Soil biology and biochemistry. Volume 136(2019)
• Publication Date: 2019-09
• Subjects: Elevated temperature -- Nitrate and ammonium amendment -- Soil microbiome -- Tibetan grassland -- Global warming; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2019.107532
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Climate warming and fertilization schemes have had widespread impacts on both above- and belowground bioprocess in natural and agricultural ecosystems, especially those in sensitive and fragile areas such as the Tibetan Plateau. In this study, we evaluated the effects of amendment of different forms of nitrogen (N) on the microbial community in typical Tibetan grassland soils under ambient conditions and conditions that mimic future effects from global warming. Both high-throughput 16S rRNA gene sequencing and functional gene microarray technology were used to detect the responses of microbial phylogenetic and functional compositions to N amendment and elevated temperature. Our results indicated that sodium nitrate and ammonium sulfate divergently shaped the microbial community composition at ambient temperature. Sodium nitrate decreased the abundance of genes involved in N cycling and carbon (C) decomposition, while ammonium sulfate had no significant effect. However, these distinct effects could be overrode by elevated temperature, where it was observed that both N amendment increased the abundance of N cycling and C decomposition genes. This indicates that future warming may "mask" the effects of amendment of different forms of N in alpine grassland. It may give a new perspective for N amendment in future warming scenario. Highlights: Warming (+2 °C) increased N and C cycling functional gene abundances. Elevated temperature overrides the effects of N amendment on bacterial communities. Elevated temperature overrides the effects of N amendment on N and C cycling gene. Our results may give a new perspective for N amendment in future warming scenario.