Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin. (19th April 2018)

• Record Type: Journal Article
• Authors: Debouki-Joudi, Saoussen; Mhirsi, Sonia; Mokni-Baizig, Nehla; Ammous-Boukhris, Nihel; Mhamdi, Hayet; Gritli, Said; Gargouri, Raja Mokdad; Marzouki, Mohamed Nejib
• Other Names: Amantini Consuelo Academic Editor.
• Is Part Of: Analytical cellular pathology. Volume 2018(2018)
• Publication Date: 2018-04-19
• Subjects: Pathology, Cellular -- Periodicals; Cytology -- Periodicals; Oncology -- Periodicals; Cancer -- Cytopathology -- Periodicals; Cancer -- Cytopathology; Cytology; Oncology; Pathology, Cellular; Cell Transformation, Neoplastic; Cells -- pathology; Cytological Techniques; Genetic Techniques; Periodicals; 571.936
• DOI: 10.1155/2018/9654067
• Languages: English
• ISSNs: 2210-7177

Abstract

Abstract : Purpose . The transcription factor Krüppel-like factor 6 (KLF6) regulates various cellular functions, such as metabolism, cell proliferation, and differentiation. KLF6 plays a key role in the development and progression of multiple human cancers. Methods . Fifty primary biopsies and 10 normal nasopharyngeal mucosae were used to analyze by RT-QPCR the expression and the copy number of wtKLF6 and the spliced variants (KLF6-SV1, KLF6-SV2, and KLF6-SV3) in Tunisian patients with nasopharyngeal carcinoma. The expression analysis of E-cadherin and cyclin D1 was conducted by RT-QPCR and Western blot, respectively. Results . The wtKLF6 was significantly downexpressed in tumors compared to normal tissues (p = 0.0015 ), whereas KLF6-SV1 and KLF6-SV2 were overexpressed in tumors compared to wtKLF6 and KLF6-SV3 (p < 0.0001 ). Copy number variation was reduced in tumors compared to normal tissues (p = 0.0071 ). Interestingly, KLF6-SV1 is associated with the juvenile form (p = 0.0003 ) which is more aggressive than the adult form of NPC. Furthermore, the oncogenic variant KLF6-SV1 was overexpressed in tumors lacking the expression of E-cadherin (p = 0.0022 ) suggesting its role in metastasis and tumor progression. The wtKLF6 is associated negatively with cyclin D1 in tumor tissues (p = 0.048 ). Conclusion . The wtKLF6 was downexpressed in contrast with the oncogenic variants. Overexpression of KLF6-SV1 is associated with young patients, and loss of E-cadherin suggests that this variant correlated with the aggressiveness of NPC.