Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth. Issue 2 (20th August 2019)

• Record Type: Journal Article
• Authors: Zhang, Xi; Cui, Yaning; Yu, Meng; Su, Bodan; Gong, Wei; Balu¡ka, Franti¡ek; Komis, George; Šamaj, Jozef; Shan, Xiaoyi; Lin, Jinxing
• Is Part Of: Plant physiology. Volume 181:Issue 2(2019)
• Page Start: 480
• Page End: 498
• Publication Date: 2019-08-20
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.19.00346
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : The differential phosphorylation of nitrate transceptor NRT1.1 contributes to the modulation of lateral root development through spatiotemporal plasma membrane dynamics and cellular trafficking. Abstract: The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development by modulating nitrate-dependent basipetal auxin export and nitrate-mediated signal transduction. Here, using the Arabidopsis ( Arabidopsis thaliana ) NRT1.1 T101D phosphomimetic and NRT1.1 T101A nonphosphorylatable mutants, we found that the phosphorylation state of NRT1.1 plays a key role in NRT1.1 function during LR development. Single-particle tracking revealed that phosphorylation affected NRT1.1 spatiotemporal dynamics. The phosphomimetic NRT1.1 T101D form showed fast lateral mobility and membrane partitioning that facilitated auxin flux under low-nitrate conditions. By contrast, nonphosphorylatable NRT1.1 T101A showed low lateral mobility and oligomerized at the plasma membrane (PM), where it induced endocytosis via the clathrin-mediated endocytosis and microdomain-mediated endocytosis pathways under high-nitrate conditions. These behaviors promoted LR development by suppressing NRT1.1-controlled auxin transport on the PM and stimulating Ca 2+ -ARABIDOPSIS NITRATE REGULATED1 signaling from the endosome.