Nitro-Fatty Acids in Plant Signaling: Nitro-Linolenic Acid Induces the Molecular Chaperone Network in Arabidopsis. Issue 2 (1st December 2015)

• Record Type: Journal Article
• Authors: Mata-Pérez, Capilla; Sánchez-Calvo, Beatriz; Padilla, María N.; Begara-Morales, Juan C.; Luque, Francisco; Melguizo, Manuel; Jiménez-Ruiz, Jaime; Fierro-Risco, Jesús; Peñas-Sanjuán, Antonio; Valderrama, Raquel; Corpas, Francisco J.; Barroso, Juan B.
• Is Part Of: Plant physiology. Volume 170:Issue 2(2016)
• Page Start: 686
• Page End: 701
• Publication Date: 2015-12-01
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.15.01671
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Endogenous nitro-fatty acids support signaling in defense against abiotic-stress. Abstract: Nitro-fatty acids (NO2 -FAs) are the product of the reaction between reactive nitrogen species derived of nitric oxide (NO) and unsaturated fatty acids. In animal systems, NO2 -FAs are considered novel signaling mediators of cell function based on a proven antiinflammatory response. Nevertheless, the interaction of NO with fatty acids in plant systems has scarcely been studied. Here, we examine the endogenous occurrence of nitro-linolenic acid (NO2 -Ln ) in Arabidopsis and the modulation of NO2 -Ln levels throughout this plant's development by mass spectrometry. The observed levels of this NO2 -FA at picomolar concentrations suggested its role as a signaling effector of cell function. In fact, a transcriptomic analysis by RNA-seq technology established a clear signaling role for this molecule, demonstrating that NO2 -Ln was involved in plant defense response against different abiotic-stress conditions, mainly by inducing heat shock proteins and supporting a conserved mechanism of action in both animal and plant defense processes. Bioinformatics analysis revealed that NO2 -Ln was also involved in the response to oxidative stress conditions, mainly depicted by H2 O2, reactive oxygen species, and oxygen-containing compound responses, with a high induction of ascorbate peroxidase expression. Closely related to these results, NO2 -Ln levels significantly rose under several abiotic-stress conditions such as wounding or exposure to salinity, cadmium, and low temperature, thus validating the outcomes found by RNA-seq technology. Jointly, to our knowledge, these are the first results showing the endogenous presence of NO2 -Ln in Arabidopsis ( Arabidopsis thaliana ) and supporting the strong signaling role of these molecules in the defense mechanism against different abiotic-stress situations.