Genome-Wide Characterization of Maize Small RNA Loci and Their Regulation in the required to maintain repression6-1 (rmr6-1) Mutant and Long-Term Abiotic Stresses. Issue 3 (8th January 2016)

• Record Type: Journal Article
• Authors: Lunardon, Alice; Forestan, Cristian; Farinati, Silvia; Axtell, Michael J.; Varotto, Serena
• Is Part Of: Plant physiology. Volume 170:Issue 3(2016)
• Page Start: 1535
• Page End: 1548
• Publication Date: 2016-01-08
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.15.01205
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Agronomically realistic, long-term drought stress mis-regulates some miRNAs and induces the down-regulation of a set of small RNA loci in the maize leaf. Abstract: Endogenous small RNAs (sRNAs ) contribute to gene regulation and genome homeostasis, but their activities and functions are incompletely known. The maize genome has a high number of transposable elements (TEs ; almost 85%), some of which spawn abundant sRNAs . We performed sRNA and total RNA sequencing from control and abiotically stressed B73 wild-type plants and rmr6-1 mutants. RMR6 encodes the largest subunit of the RNA polymerase IV complex and is responsible for accumulation of most 24-nucleotide (nt) small interfering RNAs (siRNAs ). We identified novel MIRNA loci and verified miR399 target conservation in maize. RMR6 -dependent 23-24 nt siRNA loci were specifically enriched in the upstream region of the most highly expressed genes. Most genes misregulated in rmr6-1 did not show a significant correlation with loss of flanking siRNAs, but we identified one gene supporting existing models of direct gene regulation by TE-derived siRNAs . Long-term drought correlated with changes of miRNA and sRNA accumulation, in particular inducing down-regulation of a set of sRNA loci in the wild-typeleaf.