Origin of β-Carotene-Rich Plastoglobuli in Dunaliella bardawil. Issue 4 (24th February 2014)

• Record Type: Journal Article
• Authors: Davidi, Lital; Shimoni, Eyal; Khozin-Goldberg, Inna; Zamir, Ada; Pick, Uri
• Is Part Of: Plant physiology. Volume 164:Issue 4(2014)
• Page Start: 2139
• Page End: 2156
• Publication Date: 2014-02-24
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.113.235119
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : The alga Dunaliella bardawil synthesizes storage triacylglycerides in chloroplastic lipid globules from chloroplast membrane lipid and from lipid components in cytoplasmic lipid droplets . Abstract: The halotolerant microalgae Dunaliella bardawil accumulates under nitrogen deprivation two types of lipid droplets: plastoglobuli rich in β-carotene (βC-plastoglobuli) and cytoplasmatic lipid droplets (CLDs). We describe the isolation, composition, and origin of these lipid droplets. Plastoglobuli contain β-carotene, phytoene, and galactolipids missing in CLDs. The two preparations contain different lipid-associated proteins: major lipid droplet protein in CLD and the Prorich carotene globule protein in βC-plastoglobuli. The compositions of triglyceride (TAG) molecular species, total fatty acids, and sn -1+3 and sn -2 positions in the two lipid pools are similar, except for a small increase in palmitic acid in plastoglobuli, suggesting a common origin. The formation of CLD TAG precedes that of βC-plastoglobuli, reaching a maximum after 48 h of nitrogen deprivation and then decreasing. Palmitic acid incorporation kinetics indicated that, at early stages of nitrogen deprivation, CLD TAG is synthesized mostly from newly formed fatty acids, whereas in βC-plastoglobuli, a large part of TAG is produced from fatty acids of preformed membrane lipids. Electron microscopic analyses revealed that CLDs adhere to chloroplast envelope membranes concomitant with appearance of small βC-plastoglobuli within the chloroplast. Based on these results, we propose that CLDs in D. bardawil are produced in the endoplasmatic reticulum, whereas βC-plastoglobuli are made, in part, from hydrolysis of chloroplast membrane lipids and in part, by a continual transfer of TAG or fatty acids derived from CLD.