A Long and Reversibly Self‐Assembling 1D DNA Nanostructure Built from Triplex and Quadruplex Hybrid Tiles. Issue 16 (10th March 2021)

• Record Type: Journal Article
• Authors: Lat, Prince Kumar; Schultz, Clayton W.; Yu, Hua‐Zhong; Sen, Dipankar
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 16(2021)
• Page Start: 8722
• Page End: 8727
• Publication Date: 2021-03-10
• Subjects: DNA nanotechnology -- G-quadruplexes -- one-dimensional nanostructures -- reversibility -- triplexes; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202016668
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: We report a new DNA nanostructure, an extended 1‐dimensional composite built for the first time out of structurally robust yet conveniently disassembled DNA triple helices, interspersed with short stretches of G‐quadruplexes. These "TQ Hybrid" 1‐dimensional nanostructures require potassium ions and modestly acidic pH for their formation and are easily disassembled by changes to either of these requirements. We initially prepared and characterized a "monomeric" TQ Hybrid tile; followed by "sticky" TQ s tiles, incorporating unique guanine‐only sticky ends, that enable efficient self‐assembly via G‐quartet formation of nanostructures >150 nm in length, as seen with atomic force microscopy and transmission electron microscopy. We anticipate that such DNA TQ Hybrid structures will find unique and varied application as communication modules within larger nanostructures, and as sensors, logic gates, as well as in other aspects of DNA nanotechnology. Abstract : A new and reversible 1‐dimensional DNA nanostructure, assembled from DNA triplexes and G‐quadruplexes, is reported. These "TQ Hybrid" nanostructures require potassium ions and acidic pH to form and are easily disassembled by removal of either requirement. We anticipate that they will find unique and varied application as communication modules, sensors, logic gates, and in diverse other uses of DNA nanotechnology.