Defining natural factors that stimulate and inhibit cellulose:xyloglucan hetero‐transglucosylation. (21st January 2021)

• Record Type: Journal Article
• Authors: Herburger, Klaus; Franková, Lenka; Pičmanová, Martina; Xin, Anzhou; Meulewaeter, Frank; Hudson, Andrew; Fry, Stephen C.
• Is Part Of: Plant journal. Volume 105:Number 6(2021)
• Page Start: 1549
• Page End: 1565
• Publication Date: 2021-01-21
• Subjects: cell wall -- cellulose -- Equisetum fluviatile -- expansins -- hemicelluloses -- transglycosylation -- xyloglucan; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.15131
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: Certain transglucanases can covalently graft cellulose and mixed‐linkage β‐glucan (MLG) as donor substrates onto xyloglucan as acceptor substrate and thus exhibit cellulose:xyloglucan endotransglucosylase (CXE) and MLG:xyloglucan endotransglucosylase (MXE) activities in vivo and in vitro . However, missing information on factors that stimulate or inhibit these hetero‐transglucosylation reactions limits our insight into their biological functions. To explore factors that influence hetero‐transglucosylation, we studied Equisetum fluviatile hetero‐trans‐β‐glucanase ( Ef HTG), which exhibits both CXE and MXE activity, exceeding its xyloglucan:xyloglucan homo‐transglucosylation (XET) activity. Enzyme assays employed radiolabelled and fluorescently labelled oligomeric acceptor substrates, and were conducted in vitro and in cell walls ( in situ ). With whole denatured Equisetum cell walls as donor substrate, exogenous Ef HTG (extracted from Equisetum or produced in Pichia ) exhibited all three activities (CXE, MXE, XET) in competition with each other. Acting on pure cellulose as donor substrate, the CXE action of Pichia ‐produced Ef HTG was up to approximately 300% increased by addition of methanol‐boiled Equisetum extracts; there was no similar effect when the same enzyme acted on soluble donors (MLG or xyloglucan). The methanol‐stable factor is proposed to be expansin‐like, a suggestion supported by observations of pH dependence. Screening numerous low‐molecular‐weight compounds for hetero‐transglucanase inhibition showed that cellobiose was highly effective, inhibiting the abundant endogenous CXE and MXE (but not XET) action in Equisetum internodes. Furthermore, cellobiose retarded Equisetum stem elongation, potentially owing to its effect on hetero‐transglucosylation reactions. This work provides insight and tools to further study the role of cellulose hetero‐transglucosylation in planta by identifying factors that govern this reaction. Significance Statement: The enzyme HTG can graft segments of cellulose molecules onto xyloglucan (a hemicellulose), thereby re‐structuring the cell wall via hetero‐transglycosylation. In native walls, two endogenous hemicelluloses competed with cellulose (and with each other) as substrate. HTG more readily selected cellulose as substrate if an expansin‐enriched preparation was added. Hetero‐transglycosylation was inhibited by cellobiose – a potential tool for exploring HTG's biological functions. Interestingly, cellobiose retarded Equisetum stem elongation, suggesting a role for HTG in growth.