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A facile method for the isolation of porcine heart mitochondrial malate dehydrogenase by affinity elution chromatography on Procion Red HE3B. Issue 11 (November 1983)
Record Type:
Journal Article
Title:
A facile method for the isolation of porcine heart mitochondrial malate dehydrogenase by affinity elution chromatography on Procion Red HE3B. Issue 11 (November 1983)
Main Title:
A facile method for the isolation of porcine heart mitochondrial malate dehydrogenase by affinity elution chromatography on Procion Red HE3B
Abstract : A quick, simple method has been devised for isolating pig heart mitochondrial malate dehydrogenase in apparently homogeneous state and good yield. It entails the adsorption of the enzyme to agarose-linked Procion Red HE3B and specific elution of a ternary complex consisting of the malate dehydrogenase, NAD +, and L-malate.