Discovery of rare sulfated N-unsubstituted glucosamine based heparan sulfate analogs selectively activating chemokines. Issue 10 (28th January 2021)

• Record Type: Journal Article
• Authors: Jain, Prashant; Shanthamurthy, Chethan D.; Leviatan Ben-Arye, Shani; Woods, Robert J.; Kikkeri, Raghavendra; Padler-Karavani, Vered
• Is Part Of: Chemical science. Volume 12:Issue 10(2021)
• Page Start: 3674
• Page End: 3681
• Publication Date: 2021-01-28
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0sc05862a
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : We report the synthesis of novel HS tetrasaccharides. High throughput screening using glycan microarray and SPR identified the rare HS analog for selectively inhibiting CCL2 mediated cell migration and invasion. Abstract : Achieving selective inhibition of chemokines with structurally well-defined heparan sulfate (HS) oligosaccharides can provide important insights into cancer cell migration and metastasis. However, HS is highly heterogeneous in chemical composition, which limits its therapeutic use. Here, we report the rational design and synthesis of N -unsubstituted (NU) and N -acetylated (NA) heparan sulfate tetrasaccharides that selectively inhibit structurally homologous chemokines. HS analogs were produced by divergent synthesis, where fully protected HS tetrasaccharide precursor was subjected to selective deprotection and regioselectively O -sulfated, and O -phosphorylated to obtain 13 novel HS tetrasaccharides. HS microarray and SPR analysis with a wide range of chemokines revealed the structural significance of sulfation patterns and NU domain in chemokine activities for the first time. Particularly, HT-3, 6S-NH revealed selective recognition by CCL2 chemokine. Further systematic interrogation of the role of HT-3, 6S-NH in cancer demonstrated an effective blockade of CCL2 and its receptor CCR2 interactions, thereby impairing cancer cell proliferation, migration and invasion, a step towards designing novel drug molecules.