Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana. Issue 4 (3rd March 2021)

• Record Type: Journal Article
• Authors: Zhao, Guangdong; Zhao, Wenchun; Cui, Xuepei; Xu, Baohua; Liu, Qingxin; Li, Han; Guo, Xingqi
• Is Part Of: Archives of insect biochemistry and physiology. Volume 106:Issue 4(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-03-03
• Subjects: AccMGST2 -- expression pattern -- oxidative stress -- RNAi; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21770
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: MGST2 is a member of the MAPEG superfamily, which participates in LTC4 synthesis and plays important roles in the regulation of the oxidative stress pathway and some diseases. Here, we isolated a previously uncharacterized gene in Apis cerana cerana named AccMGST2 by reverse transcription‐polymerase chain reaction. The biological characteristics of AccMGST2 were analyzed by bioinformatics. The amino acid sequence similarity between AccMGST2 and AmMGST2 of Apis mellifera reached 96.08%. The expression characteristics of AccMGST2 were explored in several tissues. The quantitative real‐time polymerase chain reaction results showed that the AccMGST2 gene was highly expressed in the head and muscle and that AccMGST2 expression responded to oxidative stress caused by different abiotic stresses. AccMGST2 was silenced using RNA interference, which decreased the expression levels of some MAPK and antioxidant genes. Therefore, we conclude that AccMGST2 is involved in the regulation of oxidative stress in A. cerana cerana . Abstract : Environmental stress induced the expression of AccMGST2, and after AccMGST2 knockdown, the expression levels of MAPK and several antioxidant‐related genes in A. cerana cerana were downregulated. Research Highlights: We isolated and identified a previously uncharacterized gene, AccMGST2, in Apis cerana cerana . AccMGST2 is highly expressed in the head and muscles and responds to oxidative stress caused by different abiotic stresses. AccMGST2 knockdown affects the expression levels of MAPK and antioxidant‐related genes.