Supplementation of a combination of herbs improves immunity, uterine cleansing and facilitate early resumption of ovarian cyclicity: A study on post-partum dairy buffaloes. (23rd May 2021)

• Record Type: Journal Article
• Authors: Japheth, Konii Puhle; Kumaresan, Arumugam; Patbandha, Tapas Kumar; Baithalu, Rubina Kumari; Selvan, Arumugam Sakthivel; Nag, Pradeep; Manimaran, Ayyasamy; Oberoi, Parminder Singh
• Is Part Of: Journal of ethnopharmacology. Volume 272(2021)
• Publication Date: 2021-05-23
• Subjects: Indigenous technical knowledge -- Polyherbal mixture -- Immunity -- Reproduction efficiency -- Gene expression -- Buffaloes; AI Artificial insemination -- ANOVA Analysis of variance -- C control -- cDNA complementary DNA -- CXCR1 chemokine receptor type 1 -- CXCR2 chemokine receptor type 2 -- DNA deoxyribonucleic acid -- DNase deoxyribonuclease -- ELISA enzyme-linked immunosorbent assay -- GAPDH Glyceraldehyde-3-phosphate dehydrogenase -- g gram -- GRK G protein-coupled receptor kinase -- IL8 Interleukin-8 -- MHz Mega Hertz -- ml Milli litre -- mm Milli meter -- NBT Nitro toluidine blue -- ng nano gram -- Nm Nano meter -- NRC National Research Council -- OD Optical density -- PMN Polymorphonuclear -- qRT-PCR Quantitative real time Polymerase chain reaction -- RNA Ribonucleic acid -- rpm rotations per minute -- RPMI Rosewell parker memorial institute -- T treatment; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2021.113931
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: A combination of Trachyspermum ammi L., Curcuma longa L., Cuminum cyminum L., Trigonella foenum-graecum L., Foeniculum vulgare Mill., Anethum graveolens L and Zingiber officinale Roscoe is used as immunity booster and reproductive efficiency enhancing agents in folklore medicine. Aim of the study: The present study aimed to assess the immunomodulatory, uterine cleansing and reproduction enhancing effects of polyherbal mixture in post-partum buffaloes. Materials and methods: Enzyme linked immunosorbent assay (ELISA) was used to investigate the effects of polyherbal mixture feeding on for quantification of neutrophil functions and blood progesterone hormone estimation. Ultrasonography was used to assess the status of uterine involution, fluid in uterus and ovarian follicular status. Quantitative real time PCR (qRT-PCR) was used to measure the expression of chemokine genes CXCR1, CXCR2 AND IL-8 . Artificial insemination with cryopreserved semen was used to breed the animals. Reproductive efficiency parameters were assessed using standard calculation methods. Results: Neutrophil functions and transcriptional abundance of chemokine genes were significantly (P < 0.05) higher in buffaloes supplemented with polyherbal mixture compared to buffaloes in control group. The rate of cervical and uterine involution was significantly (P < 0.05) higher in treatment group compared to control group. The service period was shorter, days to first insemination was earlier and the number of services per conception was lower in buffaloes supplemented with polyherbal mixture compared to the buffaloes in control group. The proportion of buffaloes with large ovarian follicles within 28 days of post-partum was also significantly (P < 0.05) higher in treatment group compared to the control group. Conclusions: The polyherbal mixture used in the study improved the immunity of the buffaloes, facilitated early involution of cervix and uterus, efficient cleansing of lochia and improved subsequent fertility. It has the potential to be used in dairy animals for improving post-partum reproductive efficiency. Graphical abstract: Image 1