Gallic acid synergistically enhances the apoptotic ability of Abutilon indicum Linn. Stem fraction inhuman U87 glioblastoma cells. (2021)

• Record Type: Journal Article
• Authors: Musthafa, Shazia Anjum; Kasinathan, Thilakavathi; Bhattacharyya, Ritobrata; Muthu, Kesavan; Kumar, Sathish; Munuswamy-Ramanujam, Ganesh
• Is Part Of: Materials today. Volume 40(2021)Supplement Part 1
• Page Start: S216
• Page End: S223
• Publication Date: 2021
• Subjects: AI Abutilon indicum -- EA ethyl acetate -- MTT 3-(4, 5–dimethylthiazol–2–yl)–5–diphenyl tetrazolium bromide -- NCCS National Centre for Cell Sciences -- DMEM Dulbecco's Modified Eagle's Medium -- PBS Phosphate buffered saline -- FBS Fetal bovine serum -- GA Gallic acid -- DMSO Dimethyl sulfoxide -- DAPI 4, 6- diamino-2-phenylindole -- DCFDA 2, 7-dichlorofuorescin diacetate -- FITC Fluorescein isothiocyanate -- ROS Reactive oxygen species -- GBM Glioblastoma multiforme -- NMR Nuclear magnetic resonance -- FACS Fluorescence activated cell sorting -- RT PCR Reverse transcriptase polymerase chain reaction; Abutilon indicum -- Malvaceae -- Glioblastoma -- Apoptosis -- Gallic acid; Materials science -- Congresses -- Periodicals; 620.1
• DOI: 10.1016/j.matpr.2020.10.285
• Languages: English
• ISSNs: 2214-7853

Abstract

Abstract: The present study reports the ability of the medicinal plant Abutilon indicum (AI) stem extract active fractions to induce apoptosis in Human U87 glioblastoma cells. Cytotoxicity guided extraction and fractionation of AI stem extracts aided in identifying and isolating the polyphenolic compound, Gallic acid (GA). Isobologram analysis revealed that, cytotoxic activity of FrF was due to synergistic effect between GA and the other components of FrF. FrF induced apoptosis correlated with increased intracellular ROS expression and DNA damage. The study for the first time proves the synergetic effect between GA and phytoconstituents of enriched AI stem fractions.