A simple colorimetric experiment using mammalian cell culture to study metabolism. Issue 2 (17th September 2020)

• Record Type: Journal Article
• Authors: Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna
• Is Part Of: Biochemistry and molecular biology education. Volume 49:Issue 2(2021)
• Page Start: 271
• Page End: 277
• Publication Date: 2020-09-17
• Subjects: biochemistry -- cell culture -- metabolism -- upper‐division undergraduate -- UV–VIS spectroscopy; Biochemistry -- Study and teaching -- Periodicals; Molecular biology -- Study and teaching -- Periodicals; 572.071
• DOI: 10.1002/bmb.21457
• Languages: English
• ISSNs: 1470-8175

Abstract

Abstract: The goal of this laboratory exercise is to give upper‐level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3‐h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand‐alone experiment. In this experiment, students are tasked with relating the acidification of cell culture medium to metabolism in order to elucidate the mechanism of action for a compound. Students can relate their experimental results to topics covered on glycolysis and oxidative phosphorylation in upper‐level biochemistry classes as well as gain valuable experience relating metabolism to drug discovery.