Cell and extracellular matrix interaction models in benign mesothelial and malignant pleural mesothelioma cells in 2D and 3D in‐vitro. (17th December 2020)

• Record Type: Journal Article
• Authors: Jagirdar, Rajesh M.; Papazoglou, Eleftherios D.; Pitaraki, Eleanna; Kouliou, Olympia A.; Rouka, Erasmia; Giannakou, Lydia; Giannopoulos, Stefanos; Sinis, Sotirios I.; Hatzoglou, Chrissi; Gourgoulianis, Konstantinos I.; Zarogiannis, Sotirios G.
• Is Part Of: Clinical and experimental pharmacology and physiology. Volume 48:Number 4(2021)
• Page Start: 543
• Page End: 552
• Publication Date: 2020-12-17
• Subjects: 2D -- 3D -- EMT -- gel contraction -- homologous cell‐derived ECM -- mesothelioma -- spheroid invasion; Clinical pharmacology -- Periodicals; Pharmacology, Experimental -- Periodicals; Physiology, Experimental -- Periodicals; Physiology, Pathological -- Periodicals; 615.1
• DOI: 10.1111/1440-1681.13446
• Languages: English
• ISSNs: 0305-1870

Abstract

Abstract: Malignant pleural mesothelioma (MPM) is an aggressive tumour that grows in the pleural cavity. MPM spheroids released in the pleural fluid can form new tumour foci. Cell–cell, cell–extracellular matrix (ECM) interactions in 2D and 3D impact malignant cell behaviour during cell adhesion, migration, proliferation and epithelial–mesenchymal transition (EMT). In this study, epithelioid, biphasic and sarcomatoid MPM cell types as well as benign mesothelial cells were tested with regards to the above phenotypes. Fibronectin (FN) and homologous cell‐derived extracellular matrix (hcd‐ECM) treated substratum differentially affected the above phenotypes. 3D MPM spheroid invasion was higher in FN‐collagen matrices in the epithelioid and biphasic cells, while 3D cell cultures of epithelioid and sarcomatoid MPM cells in FN‐collagen showed a higher contractility compared to hcd‐ECM‐collagen. Cell aggregates demonstrated invasive behaviour in hcd‐ECM matrices alone. Our results suggest that ECM and the dimensionality affect malignant cell behaviour during cell culture studies. Abstract : Malignant cell growth was investigated in 2D and 3D setting in‐vitro. Cell‐derived ECM provides a more suitable substratum for clinically relevant malignant phenotypes in in‐vitro investigations.