Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungus Amphichorda guana. Issue 9 (18th February 2021)

• Record Type: Journal Article
• Authors: Liang, Min; Lyu, Hai-Ning; Ma, Zi-Ying; Li, Er-Wei; Cai, Lei; Yin, Wen-Bing
• Is Part Of: Organic & biomolecular chemistry. Volume 19:Issue 9(2021)
• Page Start: 1960
• Page End: 1964
• Publication Date: 2021-02-18
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d1ob00100k
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : By genome mining and LC-MS analysis, a novel cyclodepsipeptide isaridin H (1 ) and seven known analogs were isolated and genetically identified from a guanophilic fungus Amphichorda guana . Abstract : Two potential non-ribosomal peptide synthetases (NRPSs) were identified in the genome of a guanophilic fungus Amphichorda guana by bioinformatics analysis and gene knockout experiments. Liquid chromatography coupled with mass spectrometry (LC-MS) guided isolation led to the discovery of a new cyclodepsipeptide isaridin H (1 ) and seven known analogs, desmethylisaridin E (2 ), isaridin E (3 ), isariin A (4 ), iso-isariin B (5 ), iso-isariin D (6 ), isariin E (7 ), and nodupetide (8 ). The absolute configuration of isaridin H (1 ) was achieved by Marfey's method. Isaridin H (1 ) showed significant antifungal activity against Botrytis cinerea and Alternaria solani .