Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro. Issue 21 (16th February 2021)

• Record Type: Journal Article
• Authors: Lee, Joongoo; Schwarz, Kevin J.; Yu, Hao; Krüger, Antje; Anslyn, Eric V.; Ellington, Andrew D.; Moore, Jeffrey S.; Jewett, Michael C.
• Is Part Of: Chemical communications. Volume 57:Issue 21(2021)
• Page Start: 2661
• Page End: 2664
• Publication Date: 2021-02-16
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc07740b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We expand the substrate scope of ribosome-mediated incorporation to α-amino acids with a variety of fluorescent groups on the sidechain. Abstract : We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosome in vitro through genetic code reprogramming.