A novel hydrophilic fluorescent probe for Cu2+ detection and imaging in HeLa cells. Issue 17 (10th March 2021)

• Record Type: Journal Article
• Authors: Wang, Xinyu; Li, Zhuo; Nie, Jiaojiao; Wu, Liangqiang; Chen, Weihong; Qi, Shaolong; Xu, Hai; Du, Jianshi; Shan, Yaming; Yang, Qingbiao
• Is Part Of: RSC advances. Volume 11:Issue 17(2021)
• Page Start: 10264
• Page End: 10271
• Publication Date: 2021-03-10
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra09894a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Probe BNQ was successfully used for detection of exogenous Cu 2+ in cells using a rare ESDPT sensing mechanism. Abstract : Copper is an essential element in living systems and plays an important role in human physiology; therefore, methods to detect the concentration of copper ions in living organisms are important. Herein, we report a highly water-soluble naphthalimide-based fluorescent probe that can be used for the detection of Cu 2+ . The probe, BNQ, has high selectivity and sensitivity. The fluorescence intensity of the probe at 520 nm was visible to the naked eye under a UV lamp; upon the gradual addition of Cu 2+, there was a colour change from green to nearly colourless. Furthermore, the detection limit of BNQ for Cu 2+ was 45.5 nM. The detection mechanism was investigated using a Job's plot and density functional theory (DFT) calculations. In addition, owing to great biocompatibility, we were able to successfully use BNQ to detect Cu 2+ in living HeLa cells with low toxicity.