Two-site assay of human α1-fetoprotein using125I-labelled monoclonal antibodies. Issue 10 (October 1981)

• Record Type: Journal Article
• Authors: Weeks, Ian; Kemp, Heather A.; Woodhead, J. Stuart
• Is Part Of: Bioscience reports. Volume 1:Issue 10(1981)
• Page Start: 785
• Page End: 791
• Publication Date: 1981-10
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01114801
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Monoclonal antibodies to human α1 -ietoprotein (AFP) have been compared with a conventionally produced antiserum using radioimmunoassay and two-site immunoradiometric techniques. A low-affinity antibody, which proved inadequate for use in a radioimmunoassay, gave asatisfactory dose-response Curve in a rapid two-site assay. A higher-affinity antibody yielded a simple, rapid, and sensitive two-site assay suitable for routine measurement of serum AFP.