Effects of seleno-Sesbania canabina galactomannan on anti-oxidative and immune function of macrophage. (1st June 2021)

• Record Type: Journal Article
• Authors: Tao, Yuheng; Wang, Ting; Huang, Caoxing; Lai, Chenhuan; Ling, Zhe; Yong, Qiang
• Is Part Of: Carbohydrate polymers. Volume 261(2021)
• Publication Date: 2021-06-01
• Subjects: Sesbania cannabina -- Galactomannan -- Selenylation -- Anti-oxidative capacity -- Immunomodulatory activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.117833
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A new galactomannan (GM) was isolated from Sesbania cannabina seed. GM was modified by HNO3 –Na2 SeO3 method to obtain seleno-galactomannan (SeGMs). The SeGMs showed a stronger protective effect against H2 O2 -induced oxidative damage. The SeGMs have no obvious improving effect on the immunomodulatory activity. Abstract: Seleno-polysaccharides have become a major topic for research owing to their high anti-oxidative capacity and immune-enhancing activities. In this study, galactomannan (GM) was isolated from Sesbania cannabina, and next modified using HNO3 –Na2 SeO3 method to obtain six varieties of seleno-galactomannans (SeGMs). FT-IR and GPC results showed the changes in chemical structure of SeGMs, indicating successful combination of selenium and GM. By measuring superoxide dismutase and malondialdehyde, the SeGMs showed a stronger protective effect against H2 O2 -induced oxidative damage in vitro than unmodified GM using macrophage RAW264.7 cell as a model, and the effect of SeGMs-14 was prominent. However, the selenylation modification did not show any obvious effect on the immunomodulatory activity of GM, as determined by the index of tumor necrosis factor-α, interleukin-6, and interleukin-1β. Overall, the prepared SeGMs from galactomannan could potentially serve as a dietary supplement of Se or an organic antioxidant.