Insulin-dependent release of 5′-nucleotidase and alkaline phosphatase from liver plasma membranes. Issue 2 (April 1992)

• Record Type: Journal Article
• Authors: Incerpi, Sandra; Baldini, Patrizia; Bello, Mario Lo; Luly, Paolo
• Is Part Of: Bioscience reports. Volume 12:Issue 2(1992)
• Page Start: 101
• Page End: 108
• Publication Date: 1992-04
• Subjects: insulin -- phospholipase C -- 5′-nucleotidase -- alkaline phosphatase -- glycosylphosphatidylinositol -- pertussis toxin -- plasma membrane, (rat liver); Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF02351214
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Insulin treatment of isolated liver plasma membranes induced the release of 5′-nucleotidase and alkaline phosphatase. This effect was maximal at physiological hormone concentrations, being 36% and 17% for 5′-nucleotidase and alkaline phosphatase respectively, and was fully mimicked by the phosphatidylinositol specific phospholipase C (PI-PLC), thus confirming the presence of a glycosyl-phosphatidylinositol anchoring-system for these exofacial enzymatic proteins. The complete inhibition of insulin dependent enzyme release by neomycin is strongly supportive of an involvement of membrane-located PI-PLC activity. In addition, the insulin-like effect on enzyme release induced by the GTP non-hydrolysable analog, GTP-γ-S, and its sensitivity to the pertussis toxin are in favour of a mediatory role exerted by the G proteins system, in the transduction of some actions of insulin.