Biochemical changes induced by Coxsackie B4 virus in short-term culture of mouse pancreatic islets. Issue 1 (January 1985)

• Record Type: Journal Article
• Authors: Szopa, T. M.; Gamble, D. R.; Taylor, K. W.
• Is Part Of: Bioscience reports. Volume 5:Issue 1(1985)
• Page Start: 63
• Page End: 69
• Publication Date: 1985-01
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01117442
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Isolated mouse pancreatic islets were infected in vitro with two strains of Coxsackie B4 virus – a tissue culture – adapted strain and a mouse pancreas-adapted strain. Within 48 h of infection changes had occurred in the biochemical activities of islets infected with the mouse pancreas-adapted strain of virus. Basal insulin release was increased two-fold in these islets, while glucose-induced insulin secretion remained unchanged. Insulin biosynthesis was greatly reduced at a sti, mulatory concentration of glucose (20 raM), thus leading to a reduced insulin content in these islets. These effects are of importance because they demonstrate that certain strains of Coxsackie B4 virus, like encephalo-myocarditis virus, may selectively alter B-cell function in vitro.