Glycosylphosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha. (December 2000)

• Record Type: Journal Article
• Authors: Okuyama, H.; Hagiwara, S.; Okamoto, T.; Kato, K.
• Is Part Of: Biochemical Society transactions. Volume 28:Number 6(2000)
• Page Start: 589
• Page End: 590
• Publication Date: 2000-12
• Subjects: GPI anchor -- secretory protein -- suspension culture; AGP, arabinogalactan protein -- GPI, glycosylphosphatidylinositol -- PAP, purple acid phosphatase; Biochemistry -- Congresses; 572
• DOI: 10.1042/bst0280589
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : A suspension culture of the moss Marchantia polymorpha was incubated with either [ 3 H]-ethanolamine or [ 3 H]myristic acid, precursors of a glycosylphosphatidylinositol (GPI) anchor. By both of these a secretory protein of 47 kDa was consistently labelled. Amino acid composition analysis of the chromatographically purified 47-kDa protein exhibited the occurrence of ethanolamine and glucosamine at approximate molar ratios to protein of 1 and 4, respectively. Thus the secretory 47-kDa protein of M. polymorpha was concluded to be a GPI-anchored protein.