Biosynthesis of fatty acids in the docosahexaenoic acid-producing bacterium Moritella marina strain MP-I. (December 2000)

• Record Type: Journal Article
• Authors: Morita, N.; Tanaka, M.; Okuyama, H.
• Is Part Of: Biochemical Society transactions. Volume 28:Number 6(2000)
• Page Start: 943
• Page End: 945
• Publication Date: 2000-12
• Subjects: docosahexaenoic acid-biosynthetic gene cluster -- fab gene cluster -- fatty acid biosynthesis; fab fatty acid biosynthetic -- DHA, docosahexaenoic acid -- EPA, eicosapentaenoic acid -- ORF, open reading frame -- ACP, acyl carrier protein -- MCT, malonyl-CoA-ACP transacylase -- KAR, β-ketoacyl-ACP redurtase -- KAS, β-ketoacyl-ACP synthetase; Biochemistry -- Congresses; 572
• DOI: 10.1042/bst0280943
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : We have isolated the fatty acid biosynthetic ( fab ) gene cluster taking part in the synthesis of middle-chain fatty acids and a genomic segment which was homologous with the eicosapentaenoic acid-biosynthetic gene cluster from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1. This segment was presumed to include the DHA-biosynthetic gene cluster of M. marina strain MP-1. When M. marina strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA.