Intraflagellar transport motors in Caenorhabditis elegans neurons. (26th October 2004)

• Record Type: Journal Article
• Authors: Scholey, J.M.; Ou, G.; Snow, J.; Gunnarson, A.
• Is Part Of: Biochemical Society transactions. Volume 32:Number 5(2004)
• Page Start: 682
• Page End: 684
• Publication Date: 2004-10-26
• Subjects: IFT-dynein -- intraflagellar transport -- kinesin-II -- microtubule-based motility -- neuron -- Osm-3-kinesin; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0320682
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : IFT (intraflagellar transport) assembles and maintains sensory cilia on the dendritic endings of chemosensory neurons within the nematode Caenorhabditis elegans . During IFT, macromolecular protein complexes called IFT particles (which carry ciliary precursors) are moved from the base of the sensory cilium to its distal tip by anterograde IFT motors (kinesin-II and Osm-3 kinesin) and back to the base by retrograde IFT-dynein [Rosenbaum and Witman (2002) Nat. Rev. Mol. Cell Biol. 3, 813–825; Scholey (2003) Annu. Rev. Cell Dev. Biol. 19, 423–443; and Snell, Pan and Wang (2004) Cell 117, 693–697]. In the present study, we describe the protein machinery of IFT in C. elegans, which we have analysed using time-lapse fluorescence microscopy of green fluorescent protein-fusion proteins in concert with ciliary mutants.