Flow injection–chemiluminescence determination of cetirizine dihydrochloride in pharmaceuticals using tris(2, 2′‐bipyridyl)ruthenium (II)–Ag(III) complex reaction. (7th December 2020)

• Record Type: Journal Article
• Authors: Moazzam, Muhammad; Asghar, Muhammad; Yaqoob, Mohammed; Ali, Samar; Nabi, Abdul
• Is Part Of: Luminescence. Volume 36:Number 3(2021)
• Page Start: 674
• Page End: 683
• Publication Date: 2020-12-07
• Subjects: Ag(III) complex -- cetirizine dihydrochloride -- chemiluminescence -- flow injection analysis -- pharmaceuticals -- [Ru(bpy)3]2+; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.3985
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: A simple and sensitive flow injection–chemiluminescence (FI–CL) method was developed for determination of cetirizine dihydrochloride (CTZH) in pharmaceuticals. The method is primarily based on the enhancement effect of CTZH on the tris(2, 2′‐bipyridyl)ruthenium (II)–diperiodatoargentate (III) ([Ru(bpy)3 ] 2+ –Ag(III) complex) CL system in an acidic medium. The optimum investigated variables of the CL reaction were: [Ru(bpy)3 ] 2+, 50 × 10 −6 mol/L; sulfuric acid, 1.0 × 10 −3 mol/L; Ag(III) complex, 100 × 10 −6 mol/L; potassium hydroxide, 1.0 × 10 −3 mol/L; flow rate, 3.0 ml/min and sample loop volume, 300 μl. The detection and quantification limits were 2.0 × 10 −4 and 5.0 × 10 −4 mg/L ( S/N of 3 and 10) respectively with a linear calibration range of 5.0 × 10 −4 to 7.5 mg/L ( R 2 = 0.9999, n = 11), injection throughput of 110/h and the relative standard deviations of 1.5–3.5% over the range studied. The methodology was successfully applied to determine CTZH in different pharmaceutical samples and validated with a high‐performance liquid chromatography method, and resulted in the recovery of 94.6–108.6%. The probable CL reaction mechanism is described in brief. Abstract : A flow injection–chemiluminescence (FI–CL) method is developed for cetirizine dihydrochloride (CTZH) determination in pharmaceuticals using [Ru(bpy)3 ] 2+ –Ag(III) complex CL reaction. The detection and quantification limits were 2.0 × 10 −4 and 5.0 × 10 −4 ppm ( S/N of 3 and 10), respectively, with a linear calibration range of 5.0 × 10 −4 to 7.5 ppm ( R 2 = 0.9999, n = 11), injection throughput of 110/h and a relative standard deviation of 1.5–3.5% over the range studied. The % recovery was from 94.6 to 108.6%.