Chemical binding of horseradish peroxidase enzyme with poly beta‐cyclodextrin and its application as molecularly imprinted polymer for the monitoring of H2O2 in human plasma samples. Issue 5 (3rd January 2021)

• Record Type: Journal Article
• Authors: Sardaremelli, Sanam; Hasanzadeh, Mohammad; Razmi, Habib
• Is Part Of: Journal of molecular recognition. Volume 34:Issue 5(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-01-03
• Subjects: biomedical analysis -- bioreceptor -- biotechnology -- heme protein imprinting -- polymer; Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2884
• Languages: English
• ISSNs: 0952-3499

Abstract

Abstract: In this study, a selective and sensitive molecular imprinting‐based electrochemical sensors, for horseradish peroxidase (HRP) entrapment was fabricated using electro polymerization of ß‐Cyclodextrin (ß‐CD) on the surface of glassy carbon electrode. Poly beta‐cyclodextrin P(ß‐CD) provide efficient surface area for self‐immobilization of HRP as well as improve imprinting efficiency. The proposed imprinted biosensor successfully utilized for detection of HRP with excellent analytical results which linear range is 0.1 mg/mL to 10 ng/mL with LOD of 2.23 ng/mL. Furthermore, electrocatalytical activity of the prepared biosensor toward the reduction of hydrogen peroxide was investigated in the ranges of 1 to 15 μM with a detection limit of 0.4 μM by using chronoamperometry technique. The developed biosensor was used for the detection of hydrogen peroxide in unprocessed human plasma sample. Abstract : In this study, a selective and sensitive molecular imprinting based electrochemical sensors, for horseradish peroxidase was fabricated using electro polymerization of β‐Cyclodextrin as cyclic oligosaccharide on the surface of glassy carbon electrode.