Monitoring the neurotransmitter release of human midbrain organoids using a redox cycling microsensor as a novel tool for personalized Parkinson's disease modelling and drug screening. Issue 7 (24th February 2021)

• Record Type: Journal Article
• Authors: Zanetti, Cristian; Spitz, Sarah; Berger, Emanuel; Bolognin, Silvia; Smits, Lisa M.; Crepaz, Philipp; Rothbauer, Mario; Rosser, Julie M.; Marchetti-Deschmann, Martina; Schwamborn, Jens C.; Ertl, Peter
• Is Part Of: Analyst. Volume 146:Issue 7(2021)
• Page Start: 2358
• Page End: 2367
• Publication Date: 2021-02-24
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an02206c
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel dopamine targeted electrochemical detection strategy has enabled the phenotyping and non-invasive monitoring of human midbrain organoids (healthy and Parkinson's diseased), by employing a redox-cycling based microsensor. Abstract : In this study, we have aimed at developing a novel electrochemical sensing approach capable of detecting dopamine, the main biomarker in Parkinson's disease, within the highly complex cell culture matrix of human midbrain organoids in a non-invasive and label-free manner. With its ability to generate organotypic structures in vitro, induced pluripotent stem cell technology has provided the basis for the development of advanced patient-derived disease models. These include models of the human midbrain, the affected region in the neurodegenerative disorder Parkinson's disease. Up to now, however, the analysis of so-called human midbrain organoids has relied on time-consuming and invasive strategies, incapable of monitoring organoid development. Using a redox-cycling approach in combination with a 3-mercaptopropionic acid self-assembled monolayer modification enabled the increase of sensor selectivity and sensitivity towards dopamine, while simultaneously reducing matrix-mediated interferences. In this work, we demonstrate the ability to detect and monitor even small differences in dopamine release between healthy and Parkinson`s disease-specific midbrain organoids over prolonged cultivation periods, which was additionally verified using liquid chromatography–multiple reaction monitoring mass spectrometry. Furthermore, the detection of a phenotypic rescue in midbrain organoids carrying a pathogenic mutation in leucine-rich repeat kinase 2, upon treatment with the leucine-rich repeat kinase 2 inhibitor II underlines the practical implementability of our sensing approach for drug screening applications as well as personalized disease modelling.