A coumarin-based "off–on" fluorescent probe for highly selective detection of hydrogen sulfide and imaging in living cells. Issue 12 (10th March 2021)

• Record Type: Journal Article
• Authors: Ou, Luying; Guo, Rui; Lin, Weiying
• Is Part Of: Analytical methods. Volume 13:Issue 12(2021)
• Page Start: 1511
• Page End: 1516
• Publication Date: 2021-03-10
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d1ay00097g
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : We designed a novel coumarin-based "off–on" fluorescent probe for detection of hydrogen sulfide changes in living cells. Abstract : Hydrogen sulfide (H2 S), as a significant signaling molecule, is associated with diverse physiological and pathological processes. However, it's still a challenge to explore outstanding tools for detecting endogenous H2 S in vivo . Thus, a simple "off–on" H2 S fluorescent probe CMHS has been reasonably designed, and it is based on coumarin as the fluorophore group. The probe CMHS displayed a crucial turn-on fluorescence enhancement (180-fold), rapid reaction time, high selectivity, and a low limit of detection (2.31 × 10 −7 M). Additionally, probe CMHS could be applied to visualize exogenous and endogenous H2 S successfully in HeLa cells with low cytotoxicity and good permeability.