Control of mRNA degradation in trypanosomes. (21st May 2008)

• Record Type: Journal Article
• Authors: Clayton, Christine; Schwede, Angela; Stewart, Mhairi; Robles, Ana; Benz, Corinna; Po, June; Wurst, Martin; Quieroz, Rafael; Archer, Stuart
• Is Part Of: Biochemical Society transactions. Volume 36:Number 3(2008)
• Page Start: 520
• Page End: 521
• Publication Date: 2008-05-21
• Subjects: kinetoplastid -- mRNA degradation -- RNA-binding protein -- RNA interference (RNAi) -- RNA recognition motif (RRM) -- trypanosome; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0360520
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Control of gene expression in trypanosomes relies almost exclusively on post-transcriptional mechanisms. Trypanosomes have the normal enzymes for mRNA decay: both the exosome and a 5′–3′-exoribonuclease are important in the degradation of very unstable transcripts, whereas the CAF1/NOT complex plays a major role in the degradation of all mRNAs tested. Targeted RNA interference screening was used to identify RNA-binding proteins that regulate mRNA degradation, and it revealed roles for proteins with RNA recognition motifs or pumilio domains.