Chenodeoxycholate Is a Potent Inducer of the Permeability Transition Pore in Rat Liver Mitochondria. Issue 1 (February 2001)

• Record Type: Journal Article
• Authors: Rolo, Anabela P.; Oliveira, Paulo J.; Moreno, António J. M.; Palmeira, Carlos M.
• Is Part Of: Bioscience reports. Volume 21:Issue 1(2001)
• Page Start: 73
• Page End: 80
• Publication Date: 2001-02
• Subjects: mitochondria -- permeability -- chenodeoxycholic acid -- calcium -- membrane potential; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1023/A:1010438202519
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Several reports support the concept that bile acids may be cytotoxic during cholestatic disease process by causing mitochondrial dysfunction. Here we report additional data and findings aimed at a better understanding of the involvement of the permeability transition pore (PTP) opening in bile acids toxicity. The mitochondrial PTP is implicated as a mediator of cell injury and death in many situations. In the presence of calcium and phosphate, chenodeoxycholic acid (CDCA) induced a permeability transition in freshly isolated rat liver mitochondria, characterized by membrane depolarization, release of matrix calcium, and osmotic swelling. All these events were blocked by cyclosporine A (CyA) and the calcium uniporter inhibitor ruthenium red (RR). The results suggest that CDCA increases the sensitivity of isolated mitochondria in vitro to the calcium-dependent induction of the PTP.