Analysing the yeast complexome—the Complex Portal rising to the challenge. Issue 6 (2nd March 2021)

• Record Type: Journal Article
• Authors: Meldal, Birgit H M; Pons, Carles; Perfetto, Livia; Del-Toro, Noemi; Wong, Edith; Aloy, Patrick; Hermjakob, Henning; Orchard, Sandra; Porras, Pablo
• Is Part Of: Nucleic acids research. Volume 49:Issue 6(2021)
• Page Start: 3156
• Page End: 3167
• Publication Date: 2021-03-02
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkab077
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: The EMBL-EBI Complex Portal is a knowledgebase of macromolecular complexes providing persistent stable identifiers. Entries are linked to literature evidence and provide details of complex membership, function, structure and complex-specific Gene Ontology annotations. Data are freely available and downloadable in HUPO-PSI community standards and missing entries can be requested for curation. In collaboration with Saccharomyces Genome Database and UniProt, the yeast complexome, a compendium of all known heteromeric assemblies from the model organism Saccharomyces cerevisiae, was curated. This expansion of knowledge and scope has led to a 50% increase in curated complexes compared to the previously published dataset, CYC2008. The yeast complexome is used as a reference resource for the analysis of complexes from large-scale experiments. Our analysis showed that genes coding for proteins in complexes tend to have more genetic interactions, are co-expressed with more genes, are more multifunctional, localize more often in the nucleus, and are more often involved in nucleic acid-related metabolic processes and processes where large machineries are the predominant functional drivers. A comparison to genetic interactions showed that about 40% of expanded co-complex pairs also have genetic interactions, suggesting strong functional links between complex members.